2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Nā mea maʻamau i hoʻohana ʻia ma ke kaʻina hoʻoponopono o ka hoʻolaha nuipa molekala pili: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Mea hana a me nā lako
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Ma keʻano holoʻokoʻa, ʻoi aku ka nui o nā waikawa amino i loko o nā huahana a Sustar ma mua o nā huahana a Zinpro.
Māhele 8 Nā hopena o ka hoʻohana ʻana
Nā hopena o nā kumu like ʻole o nā minelala trace ma ka hana hana a me ka maikaʻi o ka hua manu o nā moa waiho i ka wā hoʻomoe hope.
Kaʻina Hana
ʻenehana chelation i kuhikuhi ʻia
ʻenehana emulsification shear
ʻenehana pīpī a hoʻomaloʻo kaomi
ʻenehana hoʻomaʻalili a me ka dehumidification
ʻEnehana hoʻokele kaiapuni holomua
Pākuʻi A: Nā ʻAno Hana no ka Hoʻoholo ʻana i ka Hoʻolaha Nui o nā Peptides
Hoʻohana ʻia ke kūlana maʻamau: GB/T 22492-2008
1 Kumumanaʻo Hoʻāʻo:
Ua hoʻoholo ʻia e ka chromatography kānana gel hana kiʻekiʻe. ʻO ia hoʻi, me ka hoʻohana ʻana i ka mea hoʻopihapiha porous ma ke ʻano he pae paʻa, ma muli o ka ʻokoʻa o ka nui o ka nui o ka molekala pili o nā ʻāpana laʻana no ka hoʻokaʻawale ʻana, i ʻike ʻia ma ka pilina peptide o ka nalu absorption ultraviolet o 220nm, me ka hoʻohana ʻana i ka polokalamu hana ʻikepili i hoʻolaʻa ʻia no ka hoʻoholo ʻana i ka hoʻolaha nui o ka molekala pili e ka chromatography kānana gel (ʻo ia hoʻi, ka polokalamu GPC), ua hana ʻia nā chromatograms a me kā lākou ʻikepili, i helu ʻia e loaʻa ai ka nui o ka nui o ka molekala pili o ka peptide soybean a me ka laulā hoʻolaha.
2. Nā mea hoʻohui
Pono ka wai hoʻokolohua e hoʻokō i ka kikoʻī o ka wai lua ma GB / T6682, ʻo ka hoʻohana ʻana i nā reagents, koe wale nō nā hoʻolako kūikawā, he maʻemaʻe analytically.
2.1 ʻO nā mea hoʻohui e komo pū me ka acetonitrile (maʻemaʻe ma ke ʻano chromatographic), ka waikawa trifluoroacetic (maʻemaʻe ma ke ʻano chromatographic),
2.2 Nā mea maʻamau i hoʻohana ʻia ma ke kaʻina hoʻoponopono o ka hoʻolaha nuipa molekala pili: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Mea hana a me nā lako
3.1 Chromatograph Wai Hana Kiʻekiʻe (HPLC): kahi kahua hana chromatographic a i ʻole mea hoʻohui me kahi mea ʻike UV a me ka polokalamu hana ʻikepili GPC.
3.2 ʻĀpana kānana a me ka degassing vacuum pae kelepona.
3.3 Kaulike uila: waiwai puka 0.000 1g.
4 mau ʻanuʻu hana
4.1 Nā kūlana chromatographic a me nā hoʻokolohua hoʻololi ʻōnaehana (nā kūlana kuhikuhi)
- 4.1.1 Koluma chromatographic: TSKgelG2000swxl300 mm × 7.8 mm (ke anawaena o loko) a i ʻole nā kolamu gel ʻē aʻe o ke ʻano like me ka hana like e kūpono no ka hoʻoholo ʻana i nā protein a me nā peptides.
- 4.1.2 Pae neʻe: Acetonitrile + wai + waikawa trifluoroacetic = 20 + 80 + 0.1.
- 4.1.3 Ka nalu ʻike: 220 nm.
- 4.1.4 Ka nui o ke kahe ʻana: 0.5 mL/min.
- 4.1.5 Ka manawa ʻike: 30 min.
- 4.1.6 Ka nui o ka hoʻokomo laʻana: 20μL.
- 4.1.7 Mahana kolamu: mahana lumi.
- 4.1.8 I mea e hoʻokō ai ka ʻōnaehana chromatographic i nā koi ʻike, ua hoʻoholo ʻia ma lalo o nā kūlana chromatographic i luna, ʻo ka pono o ke kolamu chromatographic gel, ʻo ia hoʻi, ka helu kumumanaʻo o nā papa (N), ʻaʻole i emi iho ma mua o 10000 i helu ʻia ma ke kumu o nā piko o ke kūlana tripeptide (Glycine-Glycine-Glycine).
- 4.2 Ka hana ʻana o nā piʻo maʻamau o ka nuipa molekala pili
- Ua hoʻomākaukau ʻia nā hopena maʻamau peptide mass molekala pili like ʻole ma luna me ka nui o ka nui o 1 mg / mL e ka hoʻohālikelike ʻana i ka pae kelepona, i hui pū ʻia i kahi ʻāpana, a laila kānana ʻia ma o kahi membrane pae organik me ka nui o ka pore o 0.2 μm ~ 0.5 μm a hoʻokomo ʻia i loko o ka laʻana, a laila loaʻa nā chromatograms o nā kūlana. Ua loaʻa nā piʻo calibration mass molekala pili a me kā lākou mau kaulike ma ke kaha kiʻi ʻana i ka logarithm o ka nui molekala pili e kūʻē i ka manawa paʻa a i ʻole ma o ka regression linear.
4.3 Lapaʻau hāpana
E kaupaona pololei i ka 10mg o ka hāpana i loko o ka ʻōmole volumetric 10mL, e hoʻohui i kahi pae mobile liʻiliʻi, e lulu ultrasonic no 10min, i hoʻoheheʻe piha ʻia ka hāpana a hui pū ʻia, e hoʻokahe ʻia me ka pae mobile i ke unahi, a laila kānana ʻia ma o kahi membrane pae organik me ka nui o ka pore o 0.2μm ~ 0.5μm, a ua kālailai ʻia ka filtrate e like me nā kūlana chromatographic ma A.4.1.
- 5. Ka helu ʻana o ka hoʻolaha nuipa molekala pili
- Ma hope o ke kālailai ʻana i ka hopena laʻana i hoʻomākaukau ʻia ma 4.3 ma lalo o nā kūlana chromatographic o 4.1, hiki ke loaʻa ka nuipa molekala pili o ka laʻana a me kona laulā hoʻolaha ma ke pani ʻana i ka ʻikepili chromatographic o ka laʻana i loko o ka piʻo calibration 4.2 me ka polokalamu hana ʻikepili GPC. Hiki ke helu ʻia ka hoʻolaha ʻana o nā nuipa molekala pili o nā peptides like ʻole e ke ʻano normalization wahi kiʻekiʻe, e like me ke ʻano: X=A/A huina × 100
- Ma ke ʻano haʻilula: X - ʻO ka hapa nui o kahi peptide nui molekala pili i loko o ka peptide holoʻokoʻa i loko o ka hāpana, %;
- A - ʻĀpana kiʻekiʻe o kahi peptide nuipa molekala pili;
- Huina A - ka huina o nā wahi piko o kēlā me kēia peptide nuipa molekala pili, i helu ʻia i hoʻokahi wahi decimal.
- 6 Ka hana hou ʻana
- ʻAʻole e ʻoi aku ka ʻokoʻa loa ma waena o nā hoʻoholo kūʻokoʻa ʻelua i loaʻa ma lalo o nā kūlana o ka hana hou ʻana ma mua o 15% o ka awelika helu o nā hoʻoholo ʻelua.
- Pākuʻi B: Nā ʻAno Hana no ka Hoʻoholo ʻana i nā ʻAkika Amino Manuahi
- Ka hoʻohana ʻana i ke kūlana: Q/320205 KAVN05-2016
- 1.2 Nā mea hoʻohuihui a me nā mea hana
- ʻAkika ʻakika hau: maʻemaʻe ma ke ʻano loiloi
- ʻAkika perchloric: 0.0500 mol/L
- Hōʻailona: 0.1% hōʻailona poni kristal (waikawa acetic glacial)
- 2. Ka hoʻoholo ʻana o nā waikawa amino manuahi
Ua hoʻomaloʻo ʻia nā laʻana ma 80°C no 1 hola.
E kau i ka hāpana i loko o kahi pahu maloʻo e hoʻomaʻalili maoli i ka mahana o ka lumi a i ʻole e hoʻomaʻalili i lalo i kahi mahana e hiki ai ke hoʻohana.E kaupaona ma kahi o 0.1 g o ka hāpana (pololei a i 0.001 g) i loko o kahi ʻōmole conical maloʻo 250 mL.E neʻe koke i ka hana aʻe e pale aku i ka hāpana mai ka omo ʻana i ka makū o ke kaiapuniE hoʻohui i 25 mL o ka waikawa acetic glacial a kāwili maikaʻi no 5 mau minuke ʻaʻole i ʻoi aku.E hoʻohui i 2 kulu o ka hōʻailona poni kristalE hoʻohui i ka 0.0500 mol / L (±0.001) o ka hopena titration maʻamau o ka waikawa perchloric a hiki i ka loli ʻana o ka hopena mai ka poni a i ka hopena.
E hoʻopaʻa i ka nui o ka hopena maʻamau i hoʻopau ʻia.
- E hana i ka hoʻāʻo hakahaka i ka manawa like.
- 3. Ka helu ʻana a me nā hopena
- Ua hōʻike ʻia ka ʻike waikawa amino manuahi X i loko o ka reagent ma ke ʻano he ʻāpana nuipa (%) a ua helu ʻia e like me ke ʻano: X = C × (V1-V0) × 0.1445/M × 100%, ma ke ʻano:
- C - Ka nui o ka hopena waikawa perchloric maʻamau i nā mole no ka lita (mol/L)
- V1 - Ka nui i hoʻohana ʻia no ka titration o nā laʻana me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL).
- Vo - Ka nui i hoʻohana ʻia no ka titration blank me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL);
M - Ka nuipa o ka hāpana, ma nā grama (g).
| 0.1445: Ka nuipa awelika o nā waikawa amino e like me 1.00 mL o ka hopena waikawa perchloric maʻamau [c (HClO4) = 1.000 mol / L]. | 4.2.3 ʻO ka hopena titration maʻamau Cerium sulfate: ka nui c [Ce (SO4) 2] = 0.1 mol/L, i hoʻomākaukau ʻia e like me GB/T601. | |
| Ka hoʻokō ʻana i nā kūlana: Q/70920556 71-2024 | 1. Kumu hoʻoholo (ʻo Fe ma ke ʻano he laʻana) | He haʻahaʻa loa ka solubility o nā hui hao waikawa amino i loko o ka ethanol anhydrous a hiki ke hoʻoheheʻe ʻia nā ion metala manuahi i loko o ka ethanol anhydrous, ua hoʻohana ʻia ka ʻokoʻa o ka solubility ma waena o nā mea ʻelua i loko o ka ethanol anhydrous e hoʻoholo ai i ka helu chelation o nā hui hao waikawa amino. |
| Ma ke ʻano: V1 - ka nui o ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena hoʻāʻo, mL; | ʻEtanola anhydrous; ʻo ke koena e like me ka paukū 4.5.2 ma GB/T 27983-2011. | 3. Nā ʻanuʻu o ka loiloi |
| E hana i ʻelua mau hoʻokolohua ma ke ʻano like. E kaupaona i 0.1g o ka hāpana i hoʻomaloʻo ʻia ma 103±2℃ no 1 hola, pololei i 0.0001g, e hoʻohui i 100mL o ka ethanol anhydrous e hoʻoheheʻe, kānana, holoi ʻia ke koena kānana me 100mL o ka ethanol anhydrous no ka liʻiliʻi ʻekolu manawa, a laila e hoʻoili i ke koena i loko o kahi ʻōmole conical 250mL, e hoʻohui i 10mL o ka hopena waikawa sulfuric e like me ka paukū 4.5.3 ma GB/T27983-2011, a laila e hana i nā ʻanuʻu aʻe e like me ka paukū 4.5.3 "E hoʻomehana e hoʻoheheʻe a laila e hoʻomaʻalili" ma GB/T27983-2011. E hana i ka hoʻāʻo hakahaka i ka manawa like. | 4. Ka hoʻoholo ʻana i ka nui o ka hao | 4.1 ʻO ke kumumanaʻo o ka hoʻoholo ʻana ua like ia me ka paukū 4.4.1 ma GB/T 21996-2008. |
4.2. Nā Mea Hoʻohuihui a me nā Hoʻonā
| 4.2.1 ʻAkika i hui ʻia: E hoʻohui i 150mL o ka waikawa sulfuric a me 150mL o ka waikawa phosphoric i 700mL o ka wai a kāwili maikaʻi. | 4.2.2 ʻO ka hopena hōʻike sodium diphenylamine sulfonate: 5g/L, i hoʻomākaukau ʻia e like me GB/T603. | 4.2.3 ʻO ka hopena titration maʻamau Cerium sulfate: ka nui c [Ce (SO4) 2] = 0.1 mol/L, i hoʻomākaukau ʻia e like me GB/T601. | |
| 4.3 Nā ʻanuʻu o ka loiloi | E hana i ʻelua mau hoʻokolohua ma ke ʻano like. E kaupaona i ka 0.1g o ka hāpana, pololei i ka 020001g, e waiho i loko o kahi ʻōmole conical 250mL, e hoʻohui i ka 10mL o ka waikawa i hui ʻia, ma hope o ka hoʻoheheʻe ʻana, e hoʻohui i ka 30ml o ka wai a me 4 mau kulu o ka hopena hōʻike sodium dianiline sulfonate, a laila e hana i nā ʻanuʻu aʻe e like me ka paukū 4.4.2 ma GB/T21996-2008. E hana i ka hoʻāʻo hakahaka i ka manawa like. | 4.4 Hōʻike ʻana i nā hopena | ʻO ka nui o ka hao X1 o nā hui hao waikawa amino ma ke ʻano o ka hapa nui o ka hao, ka waiwai i hōʻike ʻia ma %, ua helu ʻia e like me ke ʻano (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena blank, mL; | V0 - ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena blank, mL; | C - Ka nui maoli o ka hopena maʻamau o ka cerium sulfate, mol/L5. Ka helu ʻana o ka nui o ka hao i loko o nā chelatesʻO ka nui o ka hao X2 i loko o ka chelate ma ke ʻano o ka hapa nui o ka hao, ka waiwai i hōʻike ʻia ma %, ua helu ʻia e like me ke ʻano: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Ma ke ʻano: V1 - ka nui o ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena hoʻāʻo, mL; | V2 - ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena blank, mL;nom1-Mass o ka hāpana, g. E lawe i ka awelika helu o nā hopena hoʻoholo like ʻana ma ke ʻano he hopena hoʻoholo, a ʻo ka ʻokoʻa paʻa o nā hopena hoʻoholo like ʻana ʻaʻole i ʻoi aku ma mua o 0.3%. | 0.05585 - ka nui o ka hao hao i hōʻike ʻia ma nā grama e like me 1.00 mL o ka hopena maʻamau cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Mass o ka hāpana, g. E lawe i ka awelika helu o nā hopena hoʻoholo like ʻana ma ke ʻano he hopena hoʻoholo, a ʻo ka ʻokoʻa paʻa o nā hopena hoʻoholo like ʻana ʻaʻole i ʻoi aku ma mua o 0.3%. | 6. Ka helu ʻana o ka helu chelationKa helu Chelation X3, ka waiwai i hōʻike ʻia ma %, X3 = X2/X1 × 100Pākuʻi C: Nā ʻAno Hana no ka Hoʻoholo ʻana i ka helu chelation o Zinpro |
Ka hoʻohana ʻana i ke kūlana: Q/320205 KAVNO7-2016
1. Nā mea hoʻohuihui a me nā mea hana
a) ʻAkika ʻakika hau: maʻemaʻe ma ke ʻano loiloi; b) ʻAkika perchloric: 0.0500mol/L; c) Hōʻailona: 0.1% hōʻailona poni kristal (ʻakika ʻakika hau)
2. Ka hoʻoholo ʻana o nā waikawa amino manuahi
2.1 Ua hoʻomaloʻo ʻia nā hāpana ma 80°C no 1 hola.
2.2 E kau i ka hāpana i loko o kahi pahu maloʻo e hoʻomaʻalili kūlohelohe i ka mahana o ka lumi a i ʻole e hoʻomaʻalili i lalo i kahi mahana e hiki ai ke hoʻohana.
2.3 E kaupaona ma kahi o 0.1 g o ka hāpana (pololei i 0.001 g) i loko o kahi ʻōmole conical maloʻo 250 mL
2.4 E neʻe koke i ka hana aʻe e pale aku i ka hāpana mai ka omo ʻana i ka makū o ke kaiapuni.
2.5 E hoʻohui i 25mL o ka waikawa acetic glacial a kāwili maikaʻi no ʻaʻole i ʻoi aku ma mua o 5min.
2.6 E hoʻohui i 2 kulu o ka mea hōʻike poni kristal.
2.7 Titrate me 0.0500mol/L (±0.001) hopena titration maʻamau o ka waikawa perchloric a hiki i ka loli ʻana o ka hopena mai ka poni a i ka ʻōmaʻomaʻo no 15s me ka ʻole o ka hoʻololi ʻana i ke kala ma ke ʻano he kiko hopena.
2.8 E hoʻopaʻa i ka nui o ka hopena maʻamau i hoʻopau ʻia.
2.9 E hoʻokō i ka hoʻāʻo hakahaka i ka manawa like.
- 3. Ka helu ʻana a me nā hopena
- ʻŌlelo Katalonia
- Physicochemical parameters
V1 - Ka nui i hoʻohana ʻia no ka titration o nā laʻana me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL).
Vo - Ka nui i hoʻohana ʻia no ka titration blank me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Wahi kūkahi: No.147 Alanui Qingpu, Kulanakauhale ʻo Shouan, Kalana ʻo Pujiang, Kulanakauhale ʻo Chengdu, Panalāʻau ʻo Sichuan, Kina
Kelepona: 86-18880477902
Nā huahana
Nā minelala liʻiliʻi ʻole
- Nā minelala ʻokoʻa organik
- Kawahili
- Hana lawelawe i hoʻopilikino ʻia
- Nā loulou wikiwiki
ʻIkepili Hui
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Kuhalaki | Kaomi no ka nīnau | © Kuleana kope - 2010-2025: Ua mālama ʻia nā kuleana āpau. | Palapala ʻāina KA HULI MAIKAʻI LOA Kelepona |
| Kelepona | 86-18880477902 | Kawanī | Leka uila |
| 8618880477902 | Pākē | Palani | |
| Bird | Pākē | Palani | Alemania Kepania |
| Aquatic animals | Kepanī | Kolea | Apapika Helene |
| Tureke | Ikalia | ||
| Ruminant animal g/head day | January 0.75 | ʻInikonia Apikana Kuekene |
Pōlani
- Pōkē
- ʻŌlelo Katalonia
- Physicochemical parameters
Hiniki
ʻŌlelo Laosa
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
ʻO Shona
Pukalia
- Sebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Koalia
Hōlani
| Application object | ʻUru Wiekanama | Content in full-value feed (mg/kg) | Efficacy |
| Kuhalaki | ʻŌlelo Haiti | Hauka | Kinyarwanda Mona Hunakalia |
| Piglets and fattening pigs | ʻĪkbo | Kawanī | Kanākā Kama ʻŌlelo Kurdish |
| ʻŌlelo Kyrgyz | ʻŌlelo Lākni | ||
| Bird | 300～400 | 45～60 | Makekoni Mālei Mālealama |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Nolewai
- ʻŌlelo Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
ʻŌlelo Serbia
Sesoto
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
ʻO Shona
Kiniki
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Kawahili
Tajik
Kamili
Keluku
Kailani
| Application object | ʻUru Wiekanama | Content in full-value feed (mg/kg) | Efficacy |
| ʻIidī | ʻŌlelo Yoruba | Zulu | Kinyarwanda Olia ʻŌlelo Kuleke |
| ʻŌlelo Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
