Hoʻolauna i nā Peptide Trace Mineral Chelates liʻiliʻi
Māhele 1 Moʻolelo o nā Mea Hoʻohui Mineral Trace
Hiki ke puʻunaue ʻia i ʻehā mau hanauna e like me ka hoʻomohala ʻana o nā mea hoʻohui mineral trace:
ʻO ka hanauna mua: Nā paʻakai inorganic o nā minelala trace, e like me ke keleawe sulfate, ferrous sulfate, zinc oxide, a pēlā aku; ʻO ka lua o ka hanauna: Nā paʻakai waikawa organik o nā minelala trace, e like me ka ferrous lactate, ferrous fumarate, keleawe citrate, a pēlā aku; ʻO ke kolu o ka hanauna: ʻO ka papa hānai amino acid chelate o nā minelala trace, e like me ka zinc methionine, ka hao glycine a me ka zinc glycine; ʻO ka hā o ka hanauna: Nā paʻakai protein a me nā paʻakai peptide chelating liʻiliʻi o nā minelala trace, e like me ka protein keleawe, ka protein hao, ka protein zinc, ka protein manganese, ke keleawe peptide liʻiliʻi, ka hao peptide liʻiliʻi, ka peptide zinc liʻiliʻi, ka peptide manganese liʻiliʻi, a pēlā aku.
ʻO ka hanauna mua he mau minelala inorganic, a ʻo ka lua a hiki i ka hā o nā hanauna he mau minelala organik.
Māhele 2 No ke aha e koho ai i nā Peptide Chelates liʻiliʻi
Loaʻa i nā peptide chelates liʻiliʻi ka hopena penei:
1. Ke hui pū nā peptides liʻiliʻi me nā ion metala, ua waiwai lākou i nā ʻano a paʻakikī ke hoʻopiha;
2. ʻAʻole ia e hoʻokūkū me nā kahawai amino acid, ʻoi aku ka nui o nā wahi omo a me ka wikiwiki omo wikiwiki;
3. Ka emi ʻana o ka hoʻohana ʻana i ka ikehu; 4. ʻOi aku ka nui o nā waihona, ka nui o ka hoʻohana ʻana a me ka hoʻomaikaʻi nui ʻana i ka hana hana holoholona;
5. Antibacterial a me antioxidant;
6. Ka hoʻoponopono ʻana i ka pale ʻana o ka maʻi.
Ua hōʻike ʻia he nui nā noiʻi e pili ana i nā ʻano a i ʻole nā hopena o nā peptide chelates liʻiliʻi i luna nei e loaʻa iā lākou nā manaʻolana ākea a me ka hiki ke hoʻomohala, no laila ua hoʻoholo hope kā mākou hui e lawe i nā peptide chelates liʻiliʻi ma ke ʻano he kikowaena o ka noiʻi a me ka hoʻomohala ʻana i ka huahana mineral trace organik a ka hui.
Māhele 3 Ka pono o nā peptide chelates liʻiliʻi
1. Ka pilina ma waena o nā peptides, nā waikawa amino a me nā protein
ʻOi aku ke kaumaha molekala o ka protein ma mua o 10000;
ʻO ke kaumaha molekala o ka peptide he 150 ~ 10000;
ʻO nā peptides liʻiliʻi, i kapa ʻia hoʻi he peptides molekala liʻiliʻi, aia i loko o 2 ~ 4 mau waikawa amino;
ʻO ke kaumaha molekala awelika o nā waikawa amino ma kahi o 150.
2. Hoʻohui i nā hui o nā waikawa amino a me nā peptides i hoʻopili ʻia me nā metala
(1) Nā hui hoʻonohonoho i loko o nā waikawa amino
Nā hui hoʻonohonoho i loko o nā waikawa amino:
Nā hui Amino a me carboxyl ma luna o a-carbon;
Nā hui kaulahao ʻaoʻao o kekahi mau waikawa a-amino, e like me ka hui sulfhydryl o cysteine, ka hui phenolic o tyrosine a me ka hui imidazole o histidine.
(2) Ke hoʻonohonoho ʻana i nā hui i loko o nā peptides liʻiliʻi
ʻOi aku ka nui o nā hui hoʻohui o nā peptides liʻiliʻi ma mua o nā waikawa amino. Ke chelate lākou me nā ion metala, ʻoi aku ka maʻalahi o ka chelate ʻana, a hiki ke hana i ka multidentate chelation, ka mea e ʻoi aku ai ka paʻa o ka chelate.
3. Ka pono o ka huahana peptide chelate liʻiliʻi
ʻO ke kumu kumumanaʻo o ka peptide liʻiliʻi e hoʻolaha ana i ka omo ʻana o nā minelala trace
ʻO nā ʻano omo o nā peptides liʻiliʻi ke kumu kumumanaʻo no ka hoʻolaha ʻana i ka omo ʻana o nā mea trace. Wahi a ke kumumanaʻo metabolism protein kuʻuna, ʻo ka mea e pono ai nā holoholona no ka protein ʻo ia ka mea e pono ai lākou no nā waikawa amino like ʻole. Eia nō naʻe, i nā makahiki i hala iho nei, ua hōʻike nā haʻawina he ʻokoʻa ka lakio hoʻohana o nā waikawa amino i nā meaʻai mai nā kumu like ʻole, a i ka wā e hānai ʻia ai nā holoholona me ka ʻai homozygous a i ʻole ka ʻai kaulike amino acid haʻahaʻa protein, ʻaʻole hiki ke loaʻa ka hana hana maikaʻi loa (Baker, 1977; Pinchasov et al., 1990) [2,3]. No laila, ua hōʻike kekahi poʻe akamai i ka manaʻo he hiki i nā holoholona ke omo kūikawā no ka protein piha ponoʻī a i ʻole nā peptides pili. Ua ʻike mua ʻo Agar (1953) [4] hiki i ke ala ʻōpū ke omo piha a lawe i ka diglycidyl. Mai ia manawa, ua hōʻike nā mea noiʻi i kahi hoʻopaʻapaʻa hōʻoiaʻiʻo e hiki ke omo piha ʻia nā peptides liʻiliʻi, e hōʻoia ana ua lawe ʻia a omo ʻia ka glycylglycine piha; Hiki ke omo pololei ʻia ka nui o nā peptides liʻiliʻi i loko o ke kaʻapuni ʻōnaehana ma ke ʻano o nā peptides. Hara et al. Ua kuhikuhi pū ʻo (1984)[5] ʻo nā huahana hope o ka protein i loko o ke ala ʻai he mau peptides liʻiliʻi ma mua o nā waikawa amino manuahi (FAA). Hiki i nā peptides liʻiliʻi ke hele ma waena o nā pūnaewele mucosal intestinal a komo i ke kaʻapuni systemic (Le Guowei, 1996)[6].
Ka Holomua o ka Noiʻi ʻana o ka Peptide Liʻiliʻi e Paipai ana i ka Omo ʻana o nā Mineral Trace, Qiao Wei, et al.
Hoʻoili ʻia a hoʻopili ʻia nā peptide chelates liʻiliʻi ma ke ʻano o nā peptides liʻiliʻi
Wahi a ke ʻano o ka omo ʻana a me ka lawe ʻana a me nā ʻano o nā peptides liʻiliʻi, hiki ke lawe ʻia nā minelala trace chelate me nā peptides liʻiliʻi ma ke ʻano he ligands nui, ʻoi aku ka maikaʻi o ka hoʻomaikaʻi ʻana i ka ikaika olaola o nā minelala trace. (Qiao Wei, et al)
Ka Pono o nā Peptide Chelates Liʻiliʻi
1. Ke hui pū nā peptides liʻiliʻi me nā ion metala, ua waiwai lākou i nā ʻano a paʻakikī ke hoʻopiha;
2. ʻAʻole ia e hoʻokūkū me nā kahawai amino acid, ʻoi aku ka nui o nā wahi omo a me ka wikiwiki omo wikiwiki;
3. Ka emi ʻana o ka hoʻohana ʻana i ka ikehu;
4. ʻOi aku ka nui o nā waihona, ka nui o ka hoʻohana ʻana a me ka hoʻomaikaʻi nui ʻana i ka hana hana holoholona;
5. Antibacterial a me antioxidant; 6. Hoʻoponopono i ka pale ʻana o ka maʻi.
4. Hoʻomaopopo hou aku i nā peptides
ʻO wai o nā mea hoʻohana peptide ʻelua e loaʻa hou aku ka bang no ke kālā?
- Peptide hoʻopaʻa
- ʻO ka Phosphopeptide
- Nā mea hana pili
- Peptide antimicrobial
- Peptide pale kino
- ʻO Neuropeptide
- Peptide hormone
- Peptide antioxidant
- Nā peptides meaʻai
- Nā peptides hoʻāla
(1) Ka hoʻokaʻawale ʻana o nā peptides
(2) Nā hopena kino o nā peptides
- 1. Hoʻoponopono i ke kaulike o ka wai a me ka electrolyte i loko o ke kino;
- 2. Hana i nā antibodies e kū'ē i nā bacteria a me nā maʻi no ka ʻōnaehana pale e hoʻomaikaʻi i ka hana pale;
- 3. Paipai i ka ho'ōla ʻana o nā ʻeha; Hoʻoponopono wikiwiki i ka ʻeha o ka ʻiʻo epithelial.
- 4. ʻO ka hana ʻana i nā enzymes i loko o ke kino e kōkua i ka hoʻololi ʻana i ka meaʻai i ikehu;
- 5. Hoʻoponopono i nā pūnaewele, hoʻomaikaʻi i ka metabolism o nā pūnaewele, pale i ka degeneration o nā pūnaewele, a pāʻani i kahi hana i ka pale ʻana i ka maʻi kanesa;
- 6. Paipai i ka synthesis a me ka hoʻoponopono ʻana o ka protein a me nā enzymes;
- 7. He ʻelele kemika koʻikoʻi e hoʻokaʻaʻike i ka ʻike ma waena o nā pūnaewele a me nā ʻōkana;
- 8. Kāohi ʻana i nā maʻi cardiovascular a me cerebrovascular;
- 9. Hoʻoponopono i nā ʻōnaehana endocrine a me nā ʻōnaehana nerve.
- 10. Hoʻomaikaʻi i ka ʻōnaehana hoʻoheheʻe a mālama i nā maʻi gastrointestinal mau loa;
- 11. Hoʻomaikaʻi i ka maʻi diabetes, rheumatism, rheumatoid a me nā maʻi ʻē aʻe.
- 12. Hoʻopau i ka maʻi viral, anti-aging, hoʻopau ʻana i nā radical free keu i loko o ke kino.
- 13. Hoʻolaha i ka hana hematopoietic, mālama i ka anemia, pale i ka hōʻiliʻili ʻana o ka platelet, hiki ke hoʻomaikaʻi i ka hiki ke lawe i ka oxygen o nā ʻulaʻula koko.
- 14. E hakakā pololei i nā maʻi DNA a hoʻopaʻa i nā bacteria viral.
5. Hana pālua o ka meaʻai o nā peptide chelates liʻiliʻi
Komo ka peptide chelate liʻiliʻi i loko o ke keena holoʻokoʻa i loko o ke kino holoholona, aa laila e uhaʻi aunoa i ka pilina chelationi loko o ke kelepona a wāwahi i loko o nā peptide a me nā ion metala, kahi i hoʻohana ʻia e kaholoholona e pāʻani i nā hana meaʻai pālua, ʻoi aku hoʻi kake kuleana hana o ka peptide.
Hana o ka peptide liʻiliʻi
- 1.Promote protein synthesis i loko o nā ʻiʻo ʻiʻo holoholona, hōʻoluʻolu i ka apoptosis, a paipai i ka ulu ʻana o nā holoholona
- 2. Hoʻomaikaʻi i ke ʻano o ka flora ʻōpū a hoʻolaha i ke olakino ʻōpū
- 3.E hoʻolako i ka iwi kalapona a hoʻonui i ka hana o nā enzymes digestive e like me ka amylase intestinal a me ka protease
- 4. Loaʻa nā hopena koʻikoʻi anti-oxidative
- 5. Loaʻa nā waiwai anti-inflammatory
- 6.……
6. Nā pono o nā chelates peptide liʻiliʻi ma mua o nā chelates amino acid
| Nā minelala trace chelated waikawa amino | Nā minelala trace chelated peptide liʻiliʻi | |
| Kumukūʻai mea maka | He pipiʻi nā mea maka amino acid hoʻokahi | Nui nā mea maka keratin o Kina. ʻO ka lauoho, nā māiʻuʻu a me nā pepeiaohao i ka mahi holoholona a me ka wai hoʻoheheʻe protein a me nā ʻōpala ʻili i ka ʻoihana kemika he mau mea maka protein kiʻekiʻe a makepono hoʻi. |
| Ka hopena omo ʻana | Hoʻokomo pū ʻia nā hui Amino a me carboxyl i ka chelation o nā waikawa amino a me nā mea metala i ka manawa like, e hana ana i kahi ʻano endocannabinoid bicyclic e like me nā dipeptides, me ka ʻole o nā hui carboxyl manuahi, hiki ke omo wale ʻia ma o ka ʻōnaehana oligopeptide. (Su Chunyang et al., 2002) | Ke komo nā peptides liʻiliʻi i ka chelation, hoʻokumu pinepine ʻia kahi ʻano chelation apo hoʻokahi e ka hui amino terminal a me ka oxygen bond peptide pili kokoke, a mālama ka chelate i kahi hui carboxyl manuahi, hiki ke omo ʻia ma o ka ʻōnaehana dipeptide, me ka ikaika absorption kiʻekiʻe loa ma mua o ka ʻōnaehana oligopeptide. |
| Paʻa | Nā iona metala me hoʻokahi a ʻoi aku paha mau apo ʻelima a ʻeono paha o nā hui amino, nā hui carboxyl, nā hui imidazole, nā hui phenol, a me nā hui sulfhydryl. | Ma waho aʻe o nā hui hoʻonohonoho ʻelima o nā waikawa amino, hiki i nā hui carbonyl a me imino i loko o nā peptides liʻiliʻi ke komo pū i ka hoʻonohonoho ʻana, no laila e ʻoi aku ka paʻa o nā chelates peptide liʻiliʻi ma mua o nā chelates waikawa amino.（Yang Pin et al., 2002） |
7. Nā pono o nā peptide chelates liʻiliʻi ma luna o ka glycolic acid a me nā methionine chelates
| Nā minelala trace chelated Glycine | Nā minelala trace chelated Methionine | Nā minelala trace chelated peptide liʻiliʻi | |
| Palapala hoʻonohonoho | Hiki ke hoʻohui ʻia nā hui carboxyl a me nā amino o ka glycine i nā ion metala. | Hiki ke hoʻohui ʻia nā hui carboxyl a me nā amino o ka methionine i nā ion metala. | Ke hoʻopili ʻia me nā ion metala, waiwai ia i nā ʻano hoʻonohonoho a ʻaʻole maʻalahi ka hoʻopiha ʻia. |
| Ka hana meaʻai | Hoʻokahi nā ʻano a me nā hana o nā waikawa amino. | Hoʻokahi nā ʻano a me nā hana o nā waikawa amino. | ʻO kaʻano waiwai like ʻoleʻO ka nui o nā waikawa amino e hāʻawi i ka meaʻai piha aʻe, ʻoiai hiki i nā peptides liʻiliʻi ke hana pēlā. |
| Ka hopena omo ʻana | Loaʻa i nā glycine chelatesnoaia nā hui carboxyl manuahi a loaʻa ka hopena omo lohi. | Loaʻa i nā methionine chelatesnoaia nā hui carboxyl manuahi a loaʻa ka hopena omo lohi. | Ua hoʻokumu ʻia nā peptide chelates liʻiliʻihoʻokomoke alo o nā hui carboxyl manuahi a loaʻa ka hopena omo wikiwiki. |
Māhele 4 Inoa Kālepa "Peptide-mineral Chelates liʻiliʻi"
ʻO nā Peptide-mineral Chelates liʻiliʻi, e like me ka inoa e hōʻike nei, he maʻalahi ke chelate.
Hoʻohālike ia i nā ligands peptide liʻiliʻi, ʻaʻole i maʻalahi ka hoʻomaʻemaʻe ʻia ma muli o ka nui o nā hui hoʻonohonoho, maʻalahi e hana i ka multidentate chelate me nā mea metala, me ke kūpaʻa maikaʻi.
Māhele 5 Hoʻolauna i nā huahana Peptide-mineral Chelates Series liʻiliʻi
1. ʻO ke keleawe chelated mineral peptide liʻiliʻi (inoa kālepa: Copper Amino Acid Chelate Feed Grade)
2. ʻO ka hao chelated mineral peptide liʻiliʻi (inoa kālepa: Ferrous Amino Acid Chelate Feed Grade)
3. ʻO ka peptide trace mineral chelated zinc liʻiliʻi (inoa kālepa: Zinc Amino Acid Chelate Feed Grade)
4. ʻO ka peptide trace mineral chelated manganese liʻiliʻi (inoa kālepa: Manganese Amino Acid Chelate Feed Grade)
Papa Hānai Keleawe Amino Acid Chelate
Papa Hānai Ferrous Amino Acid Chelate
Papa Hānai ʻAkika Zinc Amino Chelate
Papa hānai ʻAikawa Amino Manganese Chelate
1. Papa Hānai Keleawe Amino Acid Chelate
- Inoa Huahana: Papa Hānai Keleawe Amino Acid Chelate
- ʻAno: Nā granules ʻōmaʻomaʻo ʻeleʻele
- Nā palena kino-kemika
a) Keleawe: ≥ 10.0%
b) Huina o nā waikawa amino: ≥ 20.0%
c) Ka helu Chelation: ≥ 95%
d) ʻArsenic: ≤ 2 mg/kg
e) Ke kēpau: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Ka nui o ka wai: ≤ 5.0%
h) Fineness: Hele nā ʻāpana āpau ma o 20 mesh, me ka nui o ka ʻāpana nui o 60-80 mesh
ʻo n=0,1,2,... hōʻike i ke keleawe chelated no nā dipeptides, tripeptides, a me nā tetrapeptides
ʻO Diglycerin
ʻAno o nā peptide chelates liʻiliʻi
Nā ʻano o ke keleawe Amino Acid Chelate Feed Grade
- He huahana kēia he mineral trace organik āpau i hoʻopili ʻia e kahi kaʻina hana chelating kūikawā me nā peptides mole liʻiliʻi enzymatic mea kanu maʻemaʻe e like me nā substrates chelating a me nā mea trace.
- He paʻa kemika kēia huahana a hiki ke hōʻemi nui i kona hōʻino ʻana i nā huaora a me nā momona, a pēlā aku.
- He mea kōkua ka hoʻohana ʻana i kēia huahana i ka hoʻomaikaʻi ʻana i ka maikaʻi o ka meaʻai. Hoʻopili ʻia ka huahana ma o nā ala peptide liʻiliʻi a me nā ala amino acid, e hōʻemi ana i ka hoʻokūkū a me ka antagonism me nā mea trace ʻē aʻe, a loaʻa iā ia ka bio-absorption a me ka hoʻohana ʻana maikaʻi loa.
- ʻO ke keleawe ka ʻāpana nui o nā hunaola koko ʻulaʻula, nā ʻiʻo pili, a me nā iwi, a ua komo nā ʻano enzyme like ʻole i loko o ke kino, e hoʻoikaika i ka hana pale o ke kino, a hoʻoikaika i ka hopena antibiotic, a hiki ke hoʻonui i ka loaʻa kaumaha o kēlā me kēia lā, a hoʻomaikaʻi i ka uku hānai.
Ka hoʻohana ʻana a me ka pono o ke keleawe Amino Acid Chelate Feed Grade
| Mea noi | Ka nui i manaʻo ʻia (g/t mea waiwai piha) | ʻO ka nui o ka meaʻai waiwai piha (mg/kg) | Ka pono |
| E lūlū | 400～700 | 60～105 | 1. Hoʻomaikaʻi i ka hana hānau a me nā makahiki hoʻohana o nā puaʻa; 2. Hoʻonui i ka ikaika o nā ʻōpū a me nā puaʻa; 3. Hoʻomaikaʻi i ka palekana a me ke kū'ē ʻana i nā maʻi. |
| Puaʻa | 300～600 | 45～90 | 1. He pono no ka hoʻomaikaʻi ʻana i nā hana hematopoietic a me ka pale ʻana, e hoʻoikaika ana i ke kūpaʻa ʻana i ke kaumaha a me ke kūpaʻa ʻana i nā maʻi; 2. E hoʻonui i ka wikiwiki o ka ulu ʻana a hoʻomaikaʻi nui i ka pono o ka hānai ʻana. |
| Nā puaʻa momona | 125 | Ianuali 18.5 | |
| Manu | 125 | Ianuali 18.5 | 1. Hoʻomaikaʻi i ke kūpaʻa ʻana i ke kaumaha a hōʻemi i ka make; 2. Hoʻomaikaʻi i ka uku hānai a hoʻonui i ka wikiwiki o ka ulu ʻana. |
| Nā holoholona wai | Iʻa 40～70 | 6～10.5 | 1. Hoʻoulu i ka ulu ʻana, hoʻomaikaʻi i ka uku hānai; 2. Anti-stress, hoʻemi i ka maʻi a me ka make. |
| ʻŌpae 150～200 | 22.5～30 | ||
| Holoholona rumanina g/head lā | Ianuali 0.75 | 1. Kāohi i ka deformation hui tibial, ka maʻi neʻe "concave back", wobbler, hōʻino ʻana o nā ʻiʻo puʻuwai; 2. Kāohi i ka lauoho a i ʻole keratinization ʻaʻahu, lilo i lauoho paʻakikī, nalowale ke kuʻekuʻe maʻamau, pale i ka puka ʻana mai o nā "kiko hina" i loko o ka pōʻai maka; 3. Kāohi i ka pohō kaumaha, ka maʻi pākī, ka emi ʻana o ka hana waiū. |
2. Papa Hānai Ferrous Amino Acid Chelate
- Inoa Huahana: Ferrous Amino Acid Chelate Feed Grade
- ʻAno: Nā granules ʻōmaʻomaʻo ʻeleʻele
- Nā palena kino-kemika
a) Hao: ≥ 10.0%
b) Huina o nā waikawa amino: ≥ 19.0%
c) Ka helu Chelation: ≥ 95%
d) ʻArsenic: ≤ 2 mg/kg
e) Ke kēpau: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Ka nui o ka wai: ≤ 5.0%
h) Fineness: Hele nā ʻāpana āpau ma o 20 mesh, me ka nui o ka ʻāpana nui o 60-80 mesh
n=0,1,2,...hōʻike i ka zinc chelated no nā dipeptides, tripeptides, a me nā tetrapeptides
Nā ʻano o ka Ferrous Amino Acid Chelate Feed Grade
- He mineral trace organik kēia huahana i hoʻopili ʻia e kahi kaʻina hana chelating kūikawā me nā peptides mole liʻiliʻi enzymatic mea kanu maʻemaʻe e like me nā substrates chelating a me nā mea trace;
- He paʻa kemika kēia huahana a hiki ke hōʻemi nui i kona hōʻino ʻana i nā huaora a me nā momona, a pēlā aku. ʻO ka hoʻohana ʻana i kēia huahana he mea kōkua ia i ka hoʻomaikaʻi ʻana i ka maikaʻi o ka hānai;
- Hoʻopili ʻia ka huahana ma o nā ala peptide liʻiliʻi a me nā ala amino acid, e hōʻemi ana i ka hoʻokūkū a me ka antagonism me nā mea trace ʻē aʻe, a loaʻa iā ia ka bio-absorption a me ka hoʻohana ʻana maikaʻi loa;
- Hiki i kēia huahana ke hele ma waena o ka pale o ka placenta a me ka mammary gland, e hoʻōla i ke kino o ka fetus, hoʻonui i ke kaumaha hānau a me ke kaumaha o ka weaning, a hoʻemi i ka nui o ka make; ʻO ka hao kahi mea nui o ka hemoglobin a me ka myoglobin, hiki ke pale pono i ka anemia hemahema hao a me kona mau pilikia.
Ka hoʻohana ʻana a me ka pono o Ferrous Amino Acid Chelate Feed Grade
| Mea noi | Ka nui i manaʻo ʻia (g/t mea waiwai piha) | ʻO ka nui o ka meaʻai waiwai piha (mg/kg) | Ka pono |
| E lūlū | 300～800 | 45～120 | 1. Hoʻomaikaʻi i ka hana hānau a me ke ola hoʻohana o nā puaʻa; 2. hoʻomaikaʻi i kēlā kaumaha hānau, ke kaumaha o ka weaning a me ke ʻano like o ka puaʻa no ka hana hana maikaʻi aʻe i ka wā ma hope; 3. E hoʻomaikaʻi i ka mālama ʻana i ka hao i loko o nā puaʻa waiū a me ka nui o ka hao i loko o ka waiū e pale aku ai i ka anemia nele i ka hao i loko o nā puaʻa waiū. |
| Nā puaʻa momona a me nā puaʻa hānai | Puaʻa liʻiliʻi 300～600 | 45～90 | 1. Hoʻomaikaʻi i ka palekana o nā puaʻa, hoʻoikaika i ke kūpaʻa maʻi a hoʻomaikaʻi i ka helu ola; 2. Hoʻonui i ka wikiwiki o ka ulu ʻana, hoʻomaikaʻi i ka hoʻololi ʻana o ka hānai, hoʻonui i ke kaumaha o ka ʻōpala weaning a me ke ʻano like, a hoʻemi i ka nui o nā puaʻa maʻi; 3. Hoʻomaikaʻi i ka pae myoglobin a me ka myoglobin, pale a mālama i ka anemia hemahema hao, hoʻolilo i ka ʻili puaʻa i ʻulaʻula a hoʻomaikaʻi maopopo i ke kala o ka ʻiʻo. |
| Puaʻa momona 200～400 | 30～60 | ||
| Manu | 300～400 | 45～60 | 1. Hoʻomaikaʻi i ka hoʻololi ʻana o ka hānai, hoʻonui i ka wikiwiki o ka ulu ʻana, hoʻomaikaʻi i ka hiki ke kūʻē i ke kaumaha a hoʻemi i ka make; 2. Hoʻomaikaʻi i ka nui o ka hoʻohua ʻana i nā hua manu, hoʻemi i ka nui o ka hua manu haki a hoʻonui i ke kala o ka yolk; 3. Hoʻomaikaʻi i ka nui o ka hoʻoulu ʻana a me ka nui o ka hoʻohua ʻana o nā hua hoʻoulu a me ka nui o ke ola ʻana o nā manu ʻōpio. |
| Nā holoholona wai | 200～300 | 30～45 | 1. Hoʻoulu i ka ulu ʻana, hoʻomaikaʻi i ka hoʻololi ʻana i ka hānai; 2. Hoʻomaikaʻi i ka pale ʻana i ke kaumaha, hoʻemi i ka maʻi a me ka make. |
3. Papa Hānai ʻAkika Zinc Amino Chelate
- Inoa Huahana: Zinc Amino Acid Chelate Feed Grade
- ʻAno: nā granules brownish-melemele
- Nā palena kino-kemika
a) Zinc: ≥ 10.0%
b) Huina o nā waikawa amino: ≥ 20.5%
c) Ka helu Chelation: ≥ 95%
d) ʻArsenic: ≤ 2 mg/kg
e) Ke kēpau: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Ka nui o ka wai: ≤ 5.0%
h) Fineness: Hele nā ʻāpana āpau ma o 20 mesh, me ka nui o ka ʻāpana nui o 60-80 mesh
n=0,1,2,...hōʻike i ka zinc chelated no nā dipeptides, tripeptides, a me nā tetrapeptides
Nā ʻano o ka Zinc Amino Acid Chelate Feed Grade
He huahana kēia he mineral trace organik āpau i chelated ʻia e kahi kaʻina hana chelating kūikawā me nā peptides mole liʻiliʻi enzymatic mea kanu maʻemaʻe e like me nā substrates chelating a me nā mea trace;
He paʻa kemika kēia huahana a hiki ke hōʻemi nui i kona hōʻino ʻana i nā huaora a me nā momona, a pēlā aku.
ʻO ka hoʻohana ʻana i kēia huahana he mea kūpono ia i ka hoʻomaikaʻi ʻana i ka maikaʻi o ka hānai; Hoʻopili ʻia ka huahana ma o nā ala peptide liʻiliʻi a me nā ala amino acid, e hōʻemi ana i ka hoʻokūkū a me ka antagonism me nā mea trace ʻē aʻe, a loaʻa iā ia ka bio-absorption a me ka hoʻohana ʻana maikaʻi loa;
Hiki i kēia huahana ke hoʻomaikaʻi i ka pale ʻana, hoʻoulu i ka ulu ʻana, hoʻonui i ka hoʻololi ʻana o ka hānai a hoʻomaikaʻi i ka ʻōlinolino huluhulu;
He mea nui ka zinc ma mua o 200 mau enzymes, epithelial tissue, ribose a me gustatin. Hoʻoulu ia i ka ulu wikiwiki ʻana o nā ʻono ʻono i loko o ka mucosa alelo a hoʻoponopono i ka makemake; kāohi i nā bacteria ʻino o ka ʻōpū; a he hana antibiotics, hiki ke hoʻomaikaʻi i ka hana huna o ka ʻōnaehana hoʻoheheʻe a me ka hana o nā enzymes i loko o nā ʻiʻo a me nā cell.
Ka hoʻohana ʻana a me ka pono o ka Zinc Amino Acid Chelate Feed Grade
| Mea noi | Ka nui i manaʻo ʻia (g/t mea waiwai piha) | ʻO ka nui o ka meaʻai waiwai piha (mg/kg) | Ka pono |
| Nā puaʻa hāpai a me nā puaʻa hānai | 300～500 | 45～75 | 1. Hoʻomaikaʻi i ka hana hānau a me ke ola hoʻohana o nā puaʻa; 2. Hoʻomaikaʻi i ka ikaika o ka fetus a me nā puaʻa, hoʻoikaika i ke kū'ē ʻana i nā maʻi, a hoʻolilo iā lākou i ʻoi aku ka maikaʻi o ka hana hana ma ke kahua ma hope; 3. Hoʻomaikaʻi i ke kūlana kino o nā puaʻa hāpai a me ke kaumaha hānau o nā puaʻa liʻiliʻi. |
| Puaʻa momona, puaʻa liʻiliʻi a me nā puaʻa e ulu ana | 250～400 | 37.5～60 | 1. Hoʻomaikaʻi i ka palekana o nā puaʻa, e hōʻemi ana i ka maʻi diarrhea a me ka make; 2. Hoʻomaikaʻi i ka palatability, hoʻonui i ka ʻai ʻana i ka meaʻai, hoʻonui i ka wikiwiki o ka ulu ʻana a hoʻomaikaʻi i ka hoʻololi ʻana i ka meaʻai; 3. E hoʻomālamalama i ka hulu puaʻa a hoʻomaikaʻi i ke ʻano o ke kupapaʻu a me ka ʻiʻo. |
| Manu | 300～400 | 45～60 | 1. Hoʻomaikaʻi i ka ʻōlinolino o ka hulu manu; 2. hoʻomaikaʻi i ka helu hoʻomoe ʻana, ka helu fertilization a me ka helu hatching o nā hua hoʻohua, a hoʻoikaika i ka hiki ke kala o ka hua manu; 3. Hoʻomaikaʻi i ka hiki ke kū'ē i ke kaumaha a hoʻemi i ka make; 4. Hoʻomaikaʻi i ka hoʻololi ʻana o ka hānai a hoʻonui i ka wikiwiki o ka ulu ʻana. |
| Nā holoholona wai | Ianuali 300 | 45 | 1. Hoʻoulu i ka ulu ʻana, hoʻomaikaʻi i ka hoʻololi ʻana i ka hānai; 2. Hoʻomaikaʻi i ka pale ʻana i ke kaumaha, hoʻemi i ka maʻi a me ka make. |
| Holoholona rumanina g/head lā | 2.4 | 1. Hoʻomaikaʻi i ka hua waiū, pale i ka mastitis a me ka pala o ka foof, a hōʻemi i ka nui o nā cell somatic i loko o ka waiū; 2. Hoʻoulu i ka ulu ʻana, hoʻomaikaʻi i ka hoʻololi ʻana i ka meaʻai a hoʻomaikaʻi i ka maikaʻi o ka ʻiʻo. |
4. Manganese Amino Acid Chelate Feed Grade
- Inoa Huahana: Manganese Amino Acid Chelate Feed Grade
- ʻAno: nā granules brownish-melemele
- Nā palena kino-kemika
a) Mn: ≥ 10.0%
b) Huina o nā waikawa amino: ≥ 19.5%
c) Ka helu Chelation: ≥ 95%
d) ʻArsenic: ≤ 2 mg/kg
e) Ke kēpau: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Ka nui o ka wai: ≤ 5.0%
h) Fineness: Hele nā ʻāpana āpau ma o 20 mesh, me ka nui o ka ʻāpana nui o 60-80 mesh
n=0, 1,2,...hōʻike i ka manganese chelated no nā dipeptides, tripeptides, a me nā tetrapeptides
Nā ʻano o ka Manganese Amino Acid Chelate Feed Grade
He huahana kēia he mineral trace organik āpau i chelated ʻia e kahi kaʻina hana chelating kūikawā me nā peptides mole liʻiliʻi enzymatic mea kanu maʻemaʻe e like me nā substrates chelating a me nā mea trace;
He paʻa kemika kēia huahana a hiki ke hōʻemi nui i kona hōʻino ʻana i nā huaora a me nā momona, a pēlā aku. ʻO ka hoʻohana ʻana i kēia huahana he mea kōkua ia i ka hoʻomaikaʻi ʻana i ka maikaʻi o ka hānai;
Hoʻopili ʻia ka huahana ma o nā ala peptide liʻiliʻi a me nā ala amino acid, e hōʻemi ana i ka hoʻokūkū a me ka antagonism me nā mea trace ʻē aʻe, a loaʻa iā ia ka bio-absorption a me ka hoʻohana ʻana maikaʻi loa;
Hiki i ka huahana ke hoʻomaikaʻi i ka ulu ʻana o ka manu, hoʻomaikaʻi nui i ka hoʻololi ʻana o ka hānai a me ke kūlana olakino; a hoʻomaikaʻi i ka nui o ka hoʻomoe ʻana, ka nui o ka hoʻopuehu ʻana a me ka nui o ka moa olakino o ka manu hoʻoulu.
Pono ka manganese no ka ulu ʻana o ka iwi a me ka mālama ʻana i nā ʻiʻo pili. He pili loa ia i nā enzyme he nui; a komo i ka metabolism carbohydrate, momona a me ka protein, ka hānau hou ʻana a me ka pane ʻana o ka pale ʻana.
Ka hoʻohana ʻana a me ka pono o ka Manganese Amino Acid Chelate Feed Grade
| Mea noi | Ka nui i manaʻo ʻia (g/t mea waiwai piha) | ʻO ka nui o ka meaʻai waiwai piha (mg/kg) | Ka pono |
| Puaʻa hānau | 200～300 | 30～45 | 1. Paipai i ka ulu maʻamau o nā ʻōpū moekolohe a hoʻomaikaʻi i ka neʻe ʻana o ka sperm; 2. E hoʻomaikaʻi i ka hiki ke hānau hou o nā puaʻa hoʻoulu a hōʻemi i nā mea keakea hānau. |
| Nā puaʻa momona a me nā puaʻa hānai | 100～250 | 15～37.5 | 1. He mea pono ia e hoʻomaikaʻi i nā hana palekana, a hoʻomaikaʻi i ka hiki ke kū'ē i ke kaumaha a me ke kū'ē ʻana i nā maʻi; 2. Hoʻoulu i ka ulu ʻana a hoʻomaikaʻi nui i ka hoʻololi ʻana o ka hānai; 3. E hoʻomaikaʻi i ke kala a me ka maikaʻi o ka ʻiʻo, a hoʻomaikaʻi i ka pakeneka ʻiʻo wiwi. |
| Manu | 250～350 | 37.5～52.5 | 1. Hoʻomaikaʻi i ka hiki ke kū'ē i ke kaumaha a hoʻemi i ka make; 2. Hoʻomaikaʻi i ka helu hoʻomoe ʻana, ka helu hoʻoulu ʻana a me ka helu hoʻopuehu ʻana o nā hua hoʻohua, hoʻomaikaʻi i ka maikaʻi o ka pūpū hua a hōʻemi i ka helu haki pūpū; 3. Hoʻoulu i ka ulu ʻana o ka iwi a hoʻemi i ka nui o nā maʻi wāwae. |
| Nā holoholona wai | 100～200 | 15～30 | 1. Hoʻoulu i ka ulu ʻana a hoʻomaikaʻi i kona hiki ke kūʻē i ke kaumaha a me ke kūʻē ʻana i nā maʻi; 2. Hoʻomaikaʻi i ka neʻe ʻana o ka sperm a me ka wikiwiki o ka hoʻopuehu ʻana o nā hua i hoʻoulu ʻia. |
| Holoholona rumanina g/head lā | Pipi 1.25 | 1. Kāohi i ka maʻi hoʻohuihui momona a me ka hōʻino ʻana o ka iwi; 2. Hoʻomaikaʻi i ka hiki ke hānau hou, pale i ka hoʻopau ʻana i ka hāpai keiki a me ka maʻi lolo postpartum o nā holoholona wahine, hoʻemi i ka make ʻana o nā keiki bipi a me nā keiki hipa, a hoʻonui i ke kaumaha o nā holoholona ʻōpio i hānau hou ʻia. | |
| Kao 0.25 |
Māhele 6 FAB o nā Peptide-mineral Chelates liʻiliʻi
| S/N | F: Nā ʻano hana | A: Nā ʻokoʻa hoʻokūkū | B: Nā pono i lawe ʻia e nā ʻokoʻa hoʻokūkū i nā mea hoʻohana |
| 1 | Ka mana koho o nā mea maka | E koho i ka hydrolysis enzymatic mea kanu maʻemaʻe o nā peptides liʻiliʻi | Palekana olaola kiʻekiʻe, e pale ana i ka cannibalism |
| 2 | ʻO ka ʻenehana hoʻoheheʻe kuhikuhi no ka enzyme biological protein pālua | Ka nui o nā peptides molekala liʻiliʻi | ʻOi aku ka nui o nā "pahuhopu", ʻaʻole maʻalahi ke hoʻopiha ʻia, me ka hana olaola kiʻekiʻe a me ke kūpaʻa maikaʻi |
| 3 | ʻenehana pīpī a hoʻomaloʻo kaomi holomua | Huahana Granular, me ka nui o ka ʻāpana like, ʻoi aku ka maikaʻi o ka fluidity, ʻaʻole maʻalahi ke omo i ka makū | E hōʻoia i ka maʻalahi o ka hoʻohana ʻana, ʻoi aku ka like o ka hui ʻana i ka hānai piha |
| Ka haʻahaʻa o ka wai (≤ 5%), kahi e hōʻemi nui ai i ka mana i hoʻokumu ʻia e nā huaora a me nā hoʻomākaukau enzyme | Hoʻomaikaʻi i ke kūpaʻa o nā huahana hānai | ||
| 4 | ʻenehana kaohi hana holomua | Kaʻina hana i hoʻopaʻa piha ʻia, ke kiʻekiʻe o ka mana hoʻomalu aunoa | Kūlana palekana a paʻa hoʻi |
| 5 | ʻenehana kaohi maikaʻi kiʻekiʻe | E hoʻokumu a hoʻomaikaʻi i nā ʻano loiloi ʻepekema a me ka holomua a me nā ʻano kaohi no ka ʻike ʻana i nā mea e pili ana i ka maikaʻi o ka huahana, e like me ka protein hiki ke hoʻoheheʻe ʻia ka waikawa, ka hoʻolaha ʻana o ke kaumaha molekala, nā waikawa amino a me ka helu chelating. | E hōʻoia i ka maikaʻi, e hōʻoia i ka pono a hoʻomaikaʻi i ka pono |
Māhele 7 Hoʻohālikelike Hoʻokūkū
Kūlana VS Kūlana
Hoʻohālikelike ʻana o ka hoʻolaha peptide a me ka helu chelation o nā huahana
| Nā huahana a Sustar | Ka nui o nā peptides liʻiliʻi（180-500） | Nā huahana a Zinpro | Ka nui o nā peptides liʻiliʻi（180-500） |
| ʻAA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| ʻAA-Zn | ≥37% | AVAILA-Zn | 56% |
| Nā huahana a Sustar | Ka helu Chelation | Nā huahana a Zinpro | Ka helu Chelation |
| ʻAA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| ʻAA-Zn | 97.7% | AVAILA-Zn | 90.6% |
ʻOi aku ka haʻahaʻa o ka lakio o nā peptides liʻiliʻi o Sustar ma mua o Zinpro, a ʻoi aku ka kiʻekiʻe o ka helu chelation o nā huahana Sustar ma mua o nā huahana Zinpro.
Hoʻohālikelike ʻana i ka ʻike o 17 mau waikawa amino i nā huahana like ʻole
| Inoa o nā waikawa amino | Ke keleawe o Sustar ʻAmino Acid Chelate Papa Hānai | ʻO Zinpro Loaʻa keleawe | ʻO ka waikawa Amino Ferrous C o Sustar hānai wela Papa | Loaʻa iā Zinpro hao | Manganese a Sustar ʻAmino Acid Chelate Papa Hānai | Loaʻa iā Zinpro manganese | ʻO ka Zinc a Sustar ʻAkika Amino Papa Hānai Chelate | Loaʻa iā Zinpro kini |
| waikawa aspartic (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| waikawa glutamic (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| ʻO Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| ʻO Terionina (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| ʻArikinina (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| ʻAlanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| ʻO Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Metionina (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | Ianuali 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| ʻIsoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Huina o nā waikawa amino (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Ma keʻano holoʻokoʻa, ʻoi aku ka nui o nā waikawa amino i loko o nā huahana a Sustar ma mua o nā huahana a Zinpro.
Māhele 8 Nā hopena o ka hoʻohana ʻana
Nā hopena o nā kumu like ʻole o nā minelala trace ma ka hana hana a me ka maikaʻi o ka hua manu o nā moa waiho i ka wā hoʻomoe hope.
Kaʻina Hana
- ʻenehana chelation i kuhikuhi ʻia
- ʻenehana emulsification shear
- ʻenehana pīpī a hoʻomaloʻo kaomi
- ʻenehana hoʻomaʻalili a me ka dehumidification
- ʻEnehana hoʻokele kaiapuni holomua
Pākuʻi A: Nā ʻAno Hana no ka Hoʻoholo ʻana i ka Hoʻolaha Nui o nā Peptides
Hoʻohana ʻia ke kūlana maʻamau: GB/T 22492-2008
1 Kumumanaʻo Hoʻāʻo:
Ua hoʻoholo ʻia e ka chromatography kānana gel hana kiʻekiʻe. ʻO ia hoʻi, me ka hoʻohana ʻana i ka mea hoʻopihapiha porous ma ke ʻano he pae paʻa, ma muli o ka ʻokoʻa o ka nui o ka nui o ka molekala pili o nā ʻāpana laʻana no ka hoʻokaʻawale ʻana, i ʻike ʻia ma ka pilina peptide o ka nalu absorption ultraviolet o 220nm, me ka hoʻohana ʻana i ka polokalamu hana ʻikepili i hoʻolaʻa ʻia no ka hoʻoholo ʻana i ka hoʻolaha nui o ka molekala pili e ka chromatography kānana gel (ʻo ia hoʻi, ka polokalamu GPC), ua hana ʻia nā chromatograms a me kā lākou ʻikepili, i helu ʻia e loaʻa ai ka nui o ka nui o ka molekala pili o ka peptide soybean a me ka laulā hoʻolaha.
2. Nā mea hoʻohui
Pono ka wai hoʻokolohua e hoʻokō i ka kikoʻī o ka wai lua ma GB / T6682, ʻo ka hoʻohana ʻana i nā reagents, koe wale nō nā hoʻolako kūikawā, he maʻemaʻe analytically.
2.1 ʻO nā mea hoʻohui e komo pū me ka acetonitrile (maʻemaʻe ma ke ʻano chromatographic), ka waikawa trifluoroacetic (maʻemaʻe ma ke ʻano chromatographic),
2.2 Nā mea maʻamau i hoʻohana ʻia ma ke kaʻina hoʻoponopono o ka hoʻolaha nuipa molekala pili: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Mea hana a me nā lako
3.1 Chromatograph Wai Hana Kiʻekiʻe (HPLC): kahi kahua hana chromatographic a i ʻole mea hoʻohui me kahi mea ʻike UV a me ka polokalamu hana ʻikepili GPC.
3.2 ʻĀpana kānana a me ka degassing vacuum pae kelepona.
3.3 Kaulike uila: waiwai puka 0.000 1g.
4 mau ʻanuʻu hana
4.1 Nā kūlana chromatographic a me nā hoʻokolohua hoʻololi ʻōnaehana (nā kūlana kuhikuhi)
4.1.1 Koluma chromatographic: TSKgelG2000swxl300 mm × 7.8 mm (ke anawaena o loko) a i ʻole nā kolamu gel ʻē aʻe o ke ʻano like me ka hana like e kūpono no ka hoʻoholo ʻana i nā protein a me nā peptides.
4.1.2 Pae neʻe: Acetonitrile + wai + waikawa trifluoroacetic = 20 + 80 + 0.1.
4.1.3 Ka nalu ʻike: 220 nm.
4.1.4 Ka nui o ke kahe ʻana: 0.5 mL/min.
4.1.5 Ka manawa ʻike: 30 min.
4.1.6 Ka nui o ka hoʻokomo laʻana: 20μL.
4.1.7 Mahana kolamu: mahana lumi.
4.1.8 I mea e hoʻokō ai ka ʻōnaehana chromatographic i nā koi ʻike, ua hoʻoholo ʻia ma lalo o nā kūlana chromatographic i luna, ʻo ka pono o ke kolamu chromatographic gel, ʻo ia hoʻi, ka helu kumumanaʻo o nā papa (N), ʻaʻole i emi iho ma mua o 10000 i helu ʻia ma ke kumu o nā piko o ke kūlana tripeptide (Glycine-Glycine-Glycine).
4.2 Ka hana ʻana o nā piʻo maʻamau o ka nuipa molekala pili
Ua hoʻomākaukau ʻia nā hopena maʻamau peptide mass molekala pili like ʻole ma luna me ka nui o ka nui o 1 mg / mL e ka hoʻohālikelike ʻana i ka pae kelepona, i hui pū ʻia i kahi ʻāpana, a laila kānana ʻia ma o kahi membrane pae organik me ka nui o ka pore o 0.2 μm ~ 0.5 μm a hoʻokomo ʻia i loko o ka laʻana, a laila loaʻa nā chromatograms o nā kūlana. Ua loaʻa nā piʻo calibration mass molekala pili a me kā lākou mau kaulike ma ke kaha kiʻi ʻana i ka logarithm o ka nui molekala pili e kūʻē i ka manawa paʻa a i ʻole ma o ka regression linear.
4.3 Lapaʻau hāpana
E kaupaona pololei i ka 10mg o ka hāpana i loko o ka ʻōmole volumetric 10mL, e hoʻohui i kahi pae mobile liʻiliʻi, e lulu ultrasonic no 10min, i hoʻoheheʻe piha ʻia ka hāpana a hui pū ʻia, e hoʻokahe ʻia me ka pae mobile i ke unahi, a laila kānana ʻia ma o kahi membrane pae organik me ka nui o ka pore o 0.2μm ~ 0.5μm, a ua kālailai ʻia ka filtrate e like me nā kūlana chromatographic ma A.4.1.
5. Ka helu ʻana o ka hoʻolaha nuipa molekala pili
Ma hope o ke kālailai ʻana i ka hopena laʻana i hoʻomākaukau ʻia ma 4.3 ma lalo o nā kūlana chromatographic o 4.1, hiki ke loaʻa ka nuipa molekala pili o ka laʻana a me kona laulā hoʻolaha ma ke pani ʻana i ka ʻikepili chromatographic o ka laʻana i loko o ka piʻo calibration 4.2 me ka polokalamu hana ʻikepili GPC. Hiki ke helu ʻia ka hoʻolaha ʻana o nā nuipa molekala pili o nā peptides like ʻole e ke ʻano normalization wahi kiʻekiʻe, e like me ke ʻano: X=A/A huina × 100
Ma ke ʻano haʻilula: X - ʻO ka hapa nui o kahi peptide nui molekala pili i loko o ka peptide holoʻokoʻa i loko o ka hāpana, %;
A - ʻĀpana kiʻekiʻe o kahi peptide nuipa molekala pili;
Huina A - ka huina o nā wahi piko o kēlā me kēia peptide nuipa molekala pili, i helu ʻia i hoʻokahi wahi decimal.
6 Ka hana hou ʻana
ʻAʻole e ʻoi aku ka ʻokoʻa loa ma waena o nā hoʻoholo kūʻokoʻa ʻelua i loaʻa ma lalo o nā kūlana o ka hana hou ʻana ma mua o 15% o ka awelika helu o nā hoʻoholo ʻelua.
Pākuʻi B: Nā ʻAno Hana no ka Hoʻoholo ʻana i nā ʻAkika Amino Manuahi
Ka hoʻohana ʻana i ke kūlana: Q/320205 KAVN05-2016
1.2 Nā mea hoʻohuihui a me nā mea hana
ʻAkika ʻakika hau: maʻemaʻe ma ke ʻano loiloi
ʻAkika perchloric: 0.0500 mol/L
Hōʻailona: 0.1% hōʻailona poni kristal (waikawa acetic glacial)
2. Ka hoʻoholo ʻana o nā waikawa amino manuahi
Ua hoʻomaloʻo ʻia nā laʻana ma 80°C no 1 hola.
E kau i ka hāpana i loko o kahi pahu maloʻo e hoʻomaʻalili maoli i ka mahana o ka lumi a i ʻole e hoʻomaʻalili i lalo i kahi mahana e hiki ai ke hoʻohana.
E kaupaona ma kahi o 0.1 g o ka hāpana (pololei a i 0.001 g) i loko o kahi ʻōmole conical maloʻo 250 mL.
E neʻe koke i ka hana aʻe e pale aku i ka hāpana mai ka omo ʻana i ka makū o ke kaiapuni
E hoʻohui i 25 mL o ka waikawa acetic glacial a kāwili maikaʻi no 5 mau minuke ʻaʻole i ʻoi aku.
E hoʻohui i 2 kulu o ka hōʻailona poni kristal
E hoʻohui i ka 0.0500 mol / L (±0.001) o ka hopena titration maʻamau o ka waikawa perchloric a hiki i ka loli ʻana o ka hopena mai ka poni a i ka hopena.
E hoʻopaʻa i ka nui o ka hopena maʻamau i hoʻopau ʻia.
E hana i ka hoʻāʻo hakahaka i ka manawa like.
3. Ka helu ʻana a me nā hopena
Ua hōʻike ʻia ka ʻike waikawa amino manuahi X i loko o ka reagent ma ke ʻano he ʻāpana nuipa (%) a ua helu ʻia e like me ke ʻano: X = C × (V1-V0) × 0.1445/M × 100%, ma ke ʻano:
C - Ka nui o ka hopena waikawa perchloric maʻamau i nā mole no ka lita (mol/L)
V1 - Ka nui i hoʻohana ʻia no ka titration o nā laʻana me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL).
Vo - Ka nui i hoʻohana ʻia no ka titration blank me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL);
M - Ka nuipa o ka hāpana, ma nā grama (g).
0.1445: Ka awelika o ka nuipa o nā waikawa amino e like me 1.00 mL o ka hopena waikawa perchloric maʻamau [c (HClO4) = 1.000 mol / L].
Pākuʻi C: Nā ʻAno Hana no ka Hoʻoholo ʻana i ka helu chelation o Sustar
Ka hoʻokō ʻana i nā kūlana: Q/70920556 71-2024
1. Kumu hoʻoholo (ʻo Fe ma ke ʻano he laʻana)
He haʻahaʻa loa ka solubility o nā hui hao waikawa amino i loko o ka ethanol anhydrous a hiki ke hoʻoheheʻe ʻia nā ion metala manuahi i loko o ka ethanol anhydrous, ua hoʻohana ʻia ka ʻokoʻa o ka solubility ma waena o nā mea ʻelua i loko o ka ethanol anhydrous e hoʻoholo ai i ka helu chelation o nā hui hao waikawa amino.
2. Nā Mea Hoʻohuihui a me nā Hoʻonā
ʻEtanola anhydrous; ʻo ke koena e like me ka paukū 4.5.2 ma GB/T 27983-2011.
3. Nā ʻanuʻu o ka loiloi
E hana i ʻelua mau hoʻokolohua ma ke ʻano like. E kaupaona i 0.1g o ka hāpana i hoʻomaloʻo ʻia ma 103±2℃ no 1 hola, pololei i 0.0001g, e hoʻohui i 100mL o ka ethanol anhydrous e hoʻoheheʻe, kānana, holoi ʻia ke koena kānana me 100mL o ka ethanol anhydrous no ka liʻiliʻi ʻekolu mau manawa, a laila e hoʻoili i ke koena i loko o kahi ʻōmole conical 250mL, e hoʻohui i 10mL o ka hopena waikawa sulfuric e like me ka paukū 4.5.3 ma GB/T27983-2011, a laila e hana i nā ʻanuʻu aʻe e like me ka paukū 4.5.3 "E hoʻomehana e hoʻoheheʻe a laila e hoʻomaʻalili" ma GB/T27983-2011. E hana i ka hoʻāʻo hakahaka i ka manawa like.
4. Ka hoʻoholo ʻana i ka nui o ka hao
4.1 ʻO ke kumumanaʻo o ka hoʻoholo ʻana ua like ia me ka paukū 4.4.1 ma GB/T 21996-2008.
4.2. Nā Mea Hoʻohuihui a me nā Hoʻonā
4.2.1 ʻAkika i hui ʻia: E hoʻohui i 150mL o ka waikawa sulfuric a me 150mL o ka waikawa phosphoric i 700mL o ka wai a kāwili maikaʻi.
4.2.2 ʻO ka hopena hōʻike sodium diphenylamine sulfonate: 5g/L, i hoʻomākaukau ʻia e like me GB/T603.
4.2.3 ʻO ka hopena titration maʻamau Cerium sulfate: ka nui c [Ce (SO4) 2] = 0.1 mol/L, i hoʻomākaukau ʻia e like me GB/T601.
4.3 Nā ʻanuʻu o ka loiloi
E hana i ʻelua mau hoʻokolohua ma ke ʻano like. E kaupaona i ka 0.1g o ka hāpana, pololei i 020001g, e waiho i loko o kahi ʻōmole conical 250mL, e hoʻohui i 10mL o ka waikawa i hui ʻia, ma hope o ka hoʻoheheʻe ʻana, e hoʻohui i 30ml o ka wai a me 4 mau kulu o ka hopena hōʻike sodium dianiline sulfonate, a laila e hana i nā ʻanuʻu aʻe e like me ka paukū 4.4.2 ma GB/T21996-2008. E hana i ka hoʻāʻo hakahaka i ka manawa like.
4.4 Hōʻike ʻana i nā hopena
ʻO ka nui o ka hao X1 o nā hui hao waikawa amino ma ke ʻano o ka hapa nui o ka hao, ka waiwai i hōʻike ʻia ma %, ua helu ʻia e like me ke ʻano (1):
X1=(V-V0)×C×M×10-3×100
Ma ke ʻano: V - ka nui o ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena hoʻāʻo, mL;
V0 - ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena blank, mL;
C - Ka nui maoli o ka hopena maʻamau o ka cerium sulfate, mol/L
5. Ka helu ʻana o ka nui o ka hao i loko o nā chelates
ʻO ka nui o ka hao X2 i loko o ka chelate ma ke ʻano o ka hapa nui o ka hao, ka waiwai i hōʻike ʻia ma %, ua helu ʻia e like me ke ʻano: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
Ma ke ʻano: V1 - ka nui o ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena hoʻāʻo, mL;
V2 - ka hopena maʻamau cerium sulfate i hoʻopau ʻia no ka titration o ka hopena blank, mL;
C - Ka nui maoli o ka hopena maʻamau o ka cerium sulfate, mol/L;
0.05585 - ka nui o ka hao hao i hōʻike ʻia ma nā grama e like me 1.00 mL o ka hopena maʻamau cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass o ka hāpana, g. E lawe i ka awelika helu o nā hopena hoʻoholo like ʻana ma ke ʻano he hopena hoʻoholo, a ʻo ka ʻokoʻa paʻa o nā hopena hoʻoholo like ʻana ʻaʻole i ʻoi aku ma mua o 0.3%.
6. Ka helu ʻana o ka helu chelation
Ka helu Chelation X3, ka waiwai i hōʻike ʻia ma %, X3 = X2/X1 × 100
Pākuʻi C: Nā ʻAno Hana no ka Hoʻoholo ʻana i ka helu chelation o Zinpro
Ka hoʻohana ʻana i ke kūlana: Q/320205 KAVNO7-2016
1. Nā mea hoʻohuihui a me nā mea hana
a) ʻAkika ʻakika hau: maʻemaʻe ma ke ʻano loiloi; b) ʻAkika perchloric: 0.0500mol/L; c) Hōʻailona: 0.1% hōʻailona poni kristal (ʻakika ʻakika hau)
2. Ka hoʻoholo ʻana o nā waikawa amino manuahi
2.1 Ua hoʻomaloʻo ʻia nā hāpana ma 80°C no 1 hola.
2.2 E kau i ka hāpana i loko o kahi pahu maloʻo e hoʻomaʻalili kūlohelohe i ka mahana o ka lumi a i ʻole e hoʻomaʻalili i lalo i kahi mahana e hiki ai ke hoʻohana.
2.3 E kaupaona ma kahi o 0.1 g o ka hāpana (pololei i 0.001 g) i loko o kahi ʻōmole conical maloʻo 250 mL
2.4 E neʻe koke i ka hana aʻe e pale aku i ka hāpana mai ka omo ʻana i ka makū o ke kaiapuni.
2.5 E hoʻohui i 25mL o ka waikawa acetic glacial a kāwili maikaʻi no ʻaʻole i ʻoi aku ma mua o 5min.
2.6 E hoʻohui i 2 kulu o ka mea hōʻike poni kristal.
2.7 Titrate me 0.0500mol/L (±0.001) hopena titration maʻamau o ka waikawa perchloric a hiki i ka loli ʻana o ka hopena mai ka poni a i ka ʻōmaʻomaʻo no 15s me ka ʻole o ka hoʻololi ʻana i ke kala ma ke ʻano he kiko hopena.
2.8 E hoʻopaʻa i ka nui o ka hopena maʻamau i hoʻopau ʻia.
2.9 E hoʻokō i ka hoʻāʻo hakahaka i ka manawa like.
3. Ka helu ʻana a me nā hopena
Ua hōʻike ʻia ka ʻike waikawa amino manuahi X i loko o ka reagent ma ke ʻano he ʻāpana nuipa (%), i helu ʻia e like me ke ʻano (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
Ma ke ʻano: C - ka nui o ka hopena waikawa perchloric maʻamau i nā mole no ka lita (mol/L)
V1 - Ka nui i hoʻohana ʻia no ka titration o nā laʻana me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL).
Vo - Ka nui i hoʻohana ʻia no ka titration blank me ka hopena waikawa perchloric maʻamau, ma nā milliliters (mL);
M - Ka nuipa o ka hāpana, ma nā grama (g).
0.1445 - Ka awelika o ka nuipa o nā waikawa amino e like me 1.00 mL o ka hopena waikawa perchloric maʻamau [c (HClO4) = 1.000 mol / L].
4. Ka helu ʻana o ka helu chelation
Ua hōʻike ʻia ka helu chelation o ka hāpana ma ke ʻano he hapa nui (%), i helu ʻia e like me ke ʻano (2): helu chelation = (ka nui o ka waikawa amino - ka nui o ka waikawa amino manuahi) / ka nui o ka waikawa amino × 100%.
Ka manawa hoʻouna: Sep-17-2025
